Which scenario highlights the need for a biological spill kit?

The scenario that highlights the need for a biological spill kit involves the accidental breakage of a glass container with biohazards. This situation poses an immediate risk to health and safety, as biological materials can be hazardous if they are released into the environment. A biological spill kit is specifically designed to contain, clean up, and safely dispose of spills involving biohazardous materials. It typically includes personal protective equipment (PPE), absorbent materials, disinfectants, and tools necessary for safe cleanup, ensuring that exposure and environmental contamination are minimized.

Other scenarios, such as a power failure in the lab, a broken fume hood, or a fire alarm test, do not create an immediate biological hazard that requires the use of a biological spill kit. A power failure might affect the functioning of equipment, but it does not directly involve biological materials. Similarly, a broken fume hood compromises ventilation but does not denote a spill or exposure risk by itself. A fire alarm test is also a routine safety procedure and does not entail a biohazardous material concern. Thus, only the scenario involving the breakage of a glass container with biohazards directly concerns the critical use of a biological spill kit for managing potentially dangerous substances.